Fiorentina will be expected to reach the last 16 after Federico Bernardeschi's long-range free-kick earned the Italians a 1-0 win over Borussia Monchengladbach.
Bernardeschi gave his side an away goal to take into the return leg when he struck a contender for goal of the tournament from all of 30 yards after 43 minutes at Borussia Park on Thursday.
Roma, who beat Fiorentina 4-0 in Serie A earlier in the month, claimed a 4-0 win over Villarreal courtesy of a superbly struck first-half goal from Brazilian defender Emerson and Edin Dzeko's second-half hat-trick.
That scoreline was matched by Rostov, who raced into a 3-0 lead over Sparta Prague in the first half of their clash at Stadion Olimp-2, before Sardar Azmoun's second-half goal put the tie all but beyond doubt.
